Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.
Identifieur interne : 002B18 ( Main/Exploration ); précédent : 002B17; suivant : 002B19Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.
Auteurs : Wei Wang [République populaire de Chine] ; Lei Wei ; Anqi Yang ; Teng He ; K Y Yuen ; Cheng Chen ; Zihe RaoSource :
- Acta crystallographica. Section F, Structural biology and crystallization communications [ 1744-3091 ] ; 2009.
Descripteurs français
- KwdFr :
- Cristallisation, Cristallographie aux rayons X, Expression des gènes, Protéines de liaison à l'ARN (), Protéines de liaison à l'ARN (génétique), Protéines de liaison à l'ARN (métabolisme), Protéines virales (), Protéines virales (génétique), Protéines virales (métabolisme), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
English descriptors
- KwdEn :
- Crystallization, Crystallography, X-Ray, Gene Expression, RNA-Binding Proteins (chemistry), RNA-Binding Proteins (genetics), RNA-Binding Proteins (metabolism), SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (metabolism), Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , chemistry : RNA-Binding Proteins, Viral Proteins.
- chemical , genetics : RNA-Binding Proteins, Viral Proteins.
- chemical , metabolism : RNA-Binding Proteins, Viral Proteins.
- chemistry : SARS Virus.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Crystallization, Crystallography, X-Ray, Gene Expression.
Abstract
Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.
DOI: 10.1107/S1744309109014055
PubMed: 19407394
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001916
- to stream PubMed, to step Curation: 001916
- to stream PubMed, to step Checkpoint: 001875
- to stream Ncbi, to step Merge: 001F01
- to stream Ncbi, to step Curation: 001F01
- to stream Ncbi, to step Checkpoint: 001F01
- to stream Main, to step Merge: 002B68
- to stream Main, to step Curation: 002B18
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.</title><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><affiliation wicri:level="3"><nlm:affiliation>Tsinghua University, Beijing, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Tsinghua University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wei, Lei" sort="Wei, Lei" uniqKey="Wei L" first="Lei" last="Wei">Lei Wei</name></author><author><name sortKey="Yang, Anqi" sort="Yang, Anqi" uniqKey="Yang A" first="Anqi" last="Yang">Anqi Yang</name></author><author><name sortKey="He, Teng" sort="He, Teng" uniqKey="He T" first="Teng" last="He">Teng He</name></author><author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K Y" last="Yuen">K Y Yuen</name></author><author><name sortKey="Chen, Cheng" sort="Chen, Cheng" uniqKey="Chen C" first="Cheng" last="Chen">Cheng Chen</name></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19407394</idno><idno type="pmid">19407394</idno><idno type="doi">10.1107/S1744309109014055</idno><idno type="wicri:Area/PubMed/Corpus">001916</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001916</idno><idno type="wicri:Area/PubMed/Curation">001916</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001916</idno><idno type="wicri:Area/PubMed/Checkpoint">001875</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001875</idno><idno type="wicri:Area/Ncbi/Merge">001F01</idno><idno type="wicri:Area/Ncbi/Curation">001F01</idno><idno type="wicri:Area/Ncbi/Checkpoint">001F01</idno><idno type="wicri:Area/Main/Merge">002B68</idno><idno type="wicri:Area/Main/Curation">002B18</idno><idno type="wicri:Area/Main/Exploration">002B18</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.</title><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><affiliation wicri:level="3"><nlm:affiliation>Tsinghua University, Beijing, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Tsinghua University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wei, Lei" sort="Wei, Lei" uniqKey="Wei L" first="Lei" last="Wei">Lei Wei</name></author><author><name sortKey="Yang, Anqi" sort="Yang, Anqi" uniqKey="Yang A" first="Anqi" last="Yang">Anqi Yang</name></author><author><name sortKey="He, Teng" sort="He, Teng" uniqKey="He T" first="Teng" last="He">Teng He</name></author><author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K Y" last="Yuen">K Y Yuen</name></author><author><name sortKey="Chen, Cheng" sort="Chen, Cheng" uniqKey="Chen C" first="Cheng" last="Chen">Cheng Chen</name></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name></author></analytic><series><title level="j">Acta crystallographica. Section F, Structural biology and crystallization communications</title><idno type="eISSN">1744-3091</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Gene Expression</term><term>RNA-Binding Proteins (chemistry)</term><term>RNA-Binding Proteins (genetics)</term><term>RNA-Binding Proteins (metabolism)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Expression des gènes</term><term>Protéines de liaison à l'ARN ()</term><term>Protéines de liaison à l'ARN (génétique)</term><term>Protéines de liaison à l'ARN (métabolisme)</term><term>Protéines virales ()</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA-Binding Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA-Binding Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA-Binding Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de liaison à l'ARN</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de liaison à l'ARN</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Gene Expression</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Expression des gènes</term><term>Protéines de liaison à l'ARN</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Cheng" sort="Chen, Cheng" uniqKey="Chen C" first="Cheng" last="Chen">Cheng Chen</name><name sortKey="He, Teng" sort="He, Teng" uniqKey="He T" first="Teng" last="He">Teng He</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Wei, Lei" sort="Wei, Lei" uniqKey="Wei L" first="Lei" last="Wei">Lei Wei</name><name sortKey="Yang, Anqi" sort="Yang, Anqi" uniqKey="Yang A" first="Anqi" last="Yang">Anqi Yang</name><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K Y" last="Yuen">K Y Yuen</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B18 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B18 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19407394
|texte= Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19407394" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |